Successive DNA extractions improve characterization of soil microbial communities.

ABSTRACT: Currently, characterization of soil microbial communities relies heavily on the use of molecular approaches. Independently of the approach used, soil DNA extraction is a crucial step, and success of downstream procedures will depend on how well DNA extraction was performed. Often, studies describing and comparing soil microbial communities are based on a single DNA extraction, which may not lead to a representative recovery of DNA from all organisms present in the soil. The use of successive DNA extractions might improve soil microbial characterization, but the benefit of this approach has only been limitedly studied. To determine whether successive DNA extractions of the same soil sample would lead to different observations in terms of microbial abundance and community composition, we per

SUBMITTER: Dimitrov MR 

PROVIDER: S-EPMC5291099 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature