Longitudinal in vivo bioimaging of hepatocyte transcription factor activity following cholestatic liver injury in mice.

ABSTRACT: Molecular mechanisms regulating liver repair following cholestatic injury remain largely unknown. We have combined a mouse model of acute cholestatic liver injury, partial bile duct ligation (pBDL), with a novel longitudinal bioimaging methodology to quantify transcription factor activity during hepatic injury and repair. We administered lentiviral transcription factor activated luciferase/eGFP reporter (TFAR) cassettes to neonatal mice enabling longitudinal TFAR profiling by continued bioimaging throughout the lives of the animals and following pBDL in adulthood. Neonatal intravascular injection of VSV-G pseudotyped lentivirus resulted in almost exclusive transduction of hepatocytes allowing analysis of hepatocyte-specific transcription factor activity. We recorded acute but transient res

SUBMITTER: Delhove JM 

PROVIDER: S-EPMC5291111 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature