Comparison of Leishmania typing results obtained from 16 European clinical laboratories in 2014.

ABSTRACT: Leishmaniasis is endemic in southern Europe, and in other European countries cases are diagnosed in travellers who have visited affected areas both within the continent and beyond. Prompt and accurate diagnosis poses a challenge in clinical practice in Europe. Different methods exist for identification of the infecting Leishmania species. Sixteen clinical laboratories in 10 European countries, plus Israel and Turkey, conducted a study to assess their genotyping performance. DNA from 21 promastigote cultures of 13 species was analysed blindly by the routinely used typing method. Five different molecular targets were used, which were analysed with PCR-based methods. Different levels of identification were achieved, and either the Leishmania subgenus, species complex, or actual species were r

SUBMITTER: Van der Auwera G 

PROVIDER: S-EPMC5291127 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature