Identification and characterization of DNA sequences that prevent glucocorticoid receptor binding to nearby response elements.

ABSTRACT: Out of the myriad of potential DNA binding sites of the glucocorticoid receptor (GR) found in the human genome, only a cell-type specific minority is actually bound, indicating that the presence of a recognition sequence alone is insufficient to specify where GR binds. Cooperative interactions with other transcription factors (TFs) are known to contribute to binding specificity. Here, we reasoned that sequence signals preventing GR recruitment to certain loci provide an alternative means to confer specificity. Motif analyses uncovered candidate Negative Regulatory Sequences (NRSs) that interfere with genomic GR binding. Subsequent functional analyses demonstrated that NRSs indeed prevent GR binding to nearby response elements. We show that NRS activity is conserved across species, found in

SUBMITTER: Telorac J 

PROVIDER: S-EPMC5291246 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature