A dual transcriptional reporter and CDK-activity sensor marks cell cycle entry and progression in C. elegans.

ABSTRACT: Development, tissue homeostasis and tumor suppression depend critically on the correct regulation of cell division. Central in the cell division process is the decision whether to enter the next cell cycle and commit to going through the S and M phases, or to remain temporarily or permanently arrested. Cell cycle studies in genetic model systems could greatly benefit from visualizing cell cycle commitment in individual cells without the need of fixation. Here, we report the development and characterization of a reporter to monitor cell cycle entry in the nematode C. elegans. This reporter combines the mcm-4 promoter, to reveal Rb/E2F-mediated transcriptional control, and a live-cell sensor for CDK-activity. The CDK sensor was recently developed for use in human cells and consists of a DNA

SUBMITTER: van Rijnberk LM 

PROVIDER: S-EPMC5291519 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature