Estrogen stabilizes hypoxia-inducible factor 1α through G protein-coupled estrogen receptor 1 in eutopic endometrium of endometriosis.

ABSTRACT:

Objective

To investigate whether G protein-coupled estrogen receptor (GPER, also known as GPR30 and GPER1) stabilizes hypoxia-inducible factor 1α (HIF-1α) in eutopic endometrium (EuEM) of endometriosis.

Design

Immunohistochemical analysis and experimental in vitro study.

Setting

University hospital.

Patient(s)

Patients with or without endometriosis.

Intervention(s)

The EuEM and normal control endometrium (CoEM) were obtained by curettage. Primary cultured endometrial stromal cells (ESCs) were treated with 17β-E₂, G1, or G15.

Main outcome measure(s)

The EuEM and CoEM were collected for immunohistochemistry. Western blot, polymerase chain reaction, ELISA, and dual luciferase experiments were used to detect expression of GPER, HIF-1α, vasc

SUBMITTER: Zhang L 

PROVIDER: S-EPMC5292292 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature