Raman Spectroscopic Analyses of Jaw Periosteal Cell Mineralization.

ABSTRACT: To achieve safer patient treatments, serum-free cell culture conditions have to be established for cell therapies. In previous studies, we demonstrated that serum-free culture favored the proliferation of MSCA-1⁺ osteoprogenitors derived from the jaw periosteum. In this study, the in vitro formation of bone-specific matrix by MSCA-1⁺ jaw periosteal cells (JPCs, 3 donors) was assessed and compared under serum-free and serum-containing media conditions using the marker-free Raman spectroscopy. Based on a standard fluorescence assay, JPCs from one patient were not able to mineralize under serum-containing culture conditions, whereas the other cells showed similar mineralization levels under both conditions. Raman spectra from mineralizing MSCA-1⁺ JPCs revealed

SUBMITTER: Brauchle E 

PROVIDER: S-EPMC5292402 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature