Detecting imbalanced expression of SNP alleles by minisequencing on microarrays.

ABSTRACT:

Background

Each of the human genes or transcriptional units is likely to contain single nucleotide polymorphisms that may give rise to sequence variation between individuals and tissues on the level of RNA. Based on recent studies, differential expression of the two alleles of heterozygous coding single nucleotide polymorphisms (SNPs) may be frequent for human genes. Methods with high accuracy to be used in a high throughput setting are needed for systematic surveys of expressed sequence variation. In this study we evaluated two formats of multiplexed, microarray based minisequencing for quantitative detection of imbalanced expression of SNP alleles. We used a panel of ten SNPs located in five genes known to be expressed in two endothelial cell lines as our model system.

Results

SUBMITTER: Liljedahl U 

PROVIDER: S-EPMC529269 | biostudies-literature | 2004 Oct

REPOSITORIES: biostudies-literature