Acellular Urethra Bioscaffold: Decellularization of Whole Urethras for Tissue Engineering Applications.

ABSTRACT: Patients with stress urinary incontinence mainly suffer from malfunction of the urethra closure mechanism. We established the decellularization of porcine urethras to produce acellular urethra bioscaffolds for future tissue engineering applications, using bioscaffolds or bioscaffold-derived soluble products. Cellular removal was evaluated by H&E, DAPI and DNA quantification. The presence of specific ECM proteins was assessed through immunofluorescence staining and colorimetric assay kits. Human skeletal muscle myoblasts, muscle progenitor cells and adipose-derived stromal vascular fractions were used to evaluate the recellularization of the acellular urethra bioscaffolds. The mechanochemical decellularization system removed ~93% of tissue's DNA, generally preserving ECM's components and mi

SUBMITTER: Simoes IN 

PROVIDER: S-EPMC5292742 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature