Bystander effects elicited by single-cell photo-oxidative blue-light stimulation in retinal pigment epithelium cell networks.

ABSTRACT: 'Bystander effect' refers to the induction of biological effects in cells not directly targeted. The retinal pigment epithelium consists of hexagonal cells, forming a monolayer interconnected by gap junctions (GJs). Oxidative stress initiated in an individual cell by photostimulation (488 nm) triggered changes in reactive oxygen species (ROS), Ca²⁺ and mitochondrial membrane potential (ψ_m). The Ca²⁺ signal was transmitted to neighboring cells slowly and non-uniformly; the ROS signal spread fast and radially. Increased Ca²⁺ levels were associated with a loss in ψ_m. GJ blockers prevented the spreading of the Ca²⁺, but not the ROS-related signal. The GJ-mediated Ca²⁺ wave was associated with cell death

SUBMITTER: Ishii M 

PROVIDER: S-EPMC5292780 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature