Enhanced Development of Skeletal Myotubes from Porcine Induced Pluripotent Stem Cells.

ABSTRACT: The pig is recognized as a valuable model in biomedical research in addition to its agricultural importance. Here we describe a means for generating skeletal muscle efficiently from porcine induced pluripotent stem cells (piPSC) in vitro thereby providing a versatile platform for applications ranging from regenerative biology to the ex vivo cultivation of meat. The GSK3B inhibitor, CHIR99021 was employed to suppress apoptosis, elicit WNT signaling events and drive naïve-type piPSC along the mesoderm lineage, and, in combination with the DNA methylation inhibitor 5-aza-cytidine, to activate an early skeletal muscle transcription program. Terminal differentiation was then induced by activation of an ectopically expressed MYOD1. Myotubes, characterized by myofibril development and both sponta

SUBMITTER: Genovese NJ 

PROVIDER: S-EPMC5292944 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature