New Insights on Signal Propagation by Sensory Rhodopsin II/Transducer Complex.

ABSTRACT: The complex of two membrane proteins, sensory rhodopsin II (NpSRII) with its cognate transducer (NpHtrII), mediates negative phototaxis in halobacteria N. pharaonis. Upon light activation NpSRII triggers a signal transduction chain homologous to the two-component system in eubacterial chemotaxis. Here we report on crystal structures of the ground and active M-state of the complex in the space group I2₁2₁2₁. We demonstrate that the relative orientation of symmetrical parts of the dimer is parallel ("U"-shaped) contrary to the gusset-like ("V"-shaped) form of the previously reported structures of the NpSRII/NpHtrII complex in the space group P2₁2₁2, although the structures of the monomers taken individually are nearly the same. Computer

SUBMITTER: Ishchenko A 

PROVIDER: S-EPMC5292967 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature