Measurement of immunofunctional leptin to detect and monitor patients with functional leptin deficiency.

ABSTRACT:

Context and aims

Functional leptin deficiency is characterized by high levels of circulating immunoreactive leptin (irLep), but a reduced bioactivity of the hormone due to defective receptor binding. As a result of the fact that affected patients can be successfully treated with metreleptin, it was aimed to develop and validate a diagnostic tool to detect functional leptin deficiency.

Methods

An immunoassay capable of recognizing the functionally relevant receptor-binding complex with leptin was developed (bioLep). The analytical quality of bioLep was validated and compared to a conventional assay for immune-reactive leptin (irLep). Its clinical relevance was evaluated in a cohort of lean and obese children and adults as well as in children diagnosed with functional leptin d

SUBMITTER: Wabitsch M 

PROVIDER: S-EPMC5292973 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature