Comparison of LAMP and PCR for molecular mass screening of sand flies for Leishmania martiniquensis infection.

ABSTRACT:

Background

Leishmaniasis caused by Leishmania martiniquensis infection has been reported in human and domestic animals of Martinique Island, Germany, Switzerland, USA, Myanmar and Thailand. The peculiar clinical features of disseminated cutaneous and visceral forms co-existence render the urgent need of specific diagnostic tool to identify the natural sand fly vectors for effective prevention and control strategies. Loop-mediated isothermal amplification (LAMP) of 18S rRNA gene as well as polymerase chain reaction (PCR) of minicircle kinetoplast DNA gene (PCR-mkDNA) have never been applied to detect L. martiniquensis and L. siamensis in sand fly vectors.

Objective

The present study was aimed to validate malachite green-LAMP (MG-LAMP) and PCR-mkDNA techniques to detect L. mar

SUBMITTER: Tiwananthagorn S 

PROVIDER: S-EPMC5293119 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature