Gut microbiota inhibit Asbt-dependent intestinal bile acid reabsorption via Gata4.

ABSTRACT:

Background & aims

Regulation of bile acid homeostasis in mammals is a complex process regulated via extensive cross-talk between liver, intestine and intestinal microbiota. Here we studied the effects of gut microbiota on bile acid homeostasis in mice.

Methods

Bile acid homeostasis was assessed in four mouse models. Germfree mice, conventionally-raised mice, Asbt-KO mice and intestinal-specific Gata4-iKO mice were treated with antibiotics (bacitracin, neomycin and vancomycin; 100 mg/kg) for five days and subsequently compared with untreated mice.

Results

Attenuation of the bacterial flora by antibiotics strongly reduced fecal excretion and synthesis of bile acids, but increased the expression of the bile acid synthesis enzyme CYP7A1. Similar effects were seen in germf

SUBMITTER: Out C 

PROVIDER: S-EPMC5293168 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature