Single-Cell Characterization of 18F-FLT Uptake with Radioluminescence Microscopy.

ABSTRACT:

Unlabelled

The radiotracer 3'-deoxy-3'-(18)F-fluorothymidine ((18)F-FLT) is commonly used to measure cell proliferation in vivo. As a marker of cell proliferation, (18)F-FLT is expected to be differentially taken up by arrested and actively dividing cells, but PET measures only aggregate uptake by tumor cells and therefore the single-cell distribution of (18)F-FLT is unknown. We used a novel in vitro radioluminescence microscopy technique to measure the differential distribution of (18)F-FLT radiotracer with single-cell precision.

Methods

Using radioluminescence microscopy, we imaged the absolute uptake of (18)F-FLT in live MDA-MB-231 cells grown under different serum conditions. We then compared (18)F-FLT uptake with a standard measure of cell proliferation, using fluoresce

SUBMITTER: Sengupta D 

PROVIDER: S-EPMC5293188 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature