3D-templated, fully automated microfluidic input/output multiplexer for endocrine tissue culture and secretion sampling.

ABSTRACT: A fully automated, 16-channel microfluidic input/output multiplexer (μMUX) has been developed for interfacing to primary cells and to improve understanding of the dynamics of endocrine tissue function. The device utilizes pressure driven push-up valves for precise manipulation of nutrient input and hormone output dynamics, allowing time resolved interrogation of the cells. The ability to alternate any of the 16 channels from input to output, and vice versa, provides for high experimental flexibility without the need to alter microchannel designs. 3D-printed interface templates were custom designed to sculpt the above-channel polydimethylsiloxane (PDMS) in microdevices, creating millimeter scale reservoirs and confinement chambers to interface primary murine islets and adipose tissue explan

SUBMITTER: Li X 

PROVIDER: S-EPMC5293597 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature