Accounting for Protein Subcellular Localization: A Compartmental Map of the Rat Liver Proteome.

ABSTRACT: Accurate knowledge of the intracellular location of proteins is important for numerous areas of biomedical research including assessing fidelity of putative protein-protein interactions, modeling cellular processes at a system-wide level and investigating metabolic and disease pathways. Many proteins have not been localized, or have been incompletely localized, partly because most studies do not account for entire subcellular distribution. Thus, proteins are frequently assigned to one organelle whereas a significant fraction may reside elsewhere. As a step toward a comprehensive cellular map, we used subcellular fractionation with classic balance sheet analysis and isobaric labeling/quantitative mass spectrometry to assign locations to >6000 rat liver proteins. We provide quantitative data

SUBMITTER: Jadot M 

PROVIDER: S-EPMC5294208 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature