Enhanced cell-permeant Cre protein for site-specific recombination in cultured cells.

ABSTRACT:

Background

Cell-permeant Cre DNA site-specific recombinases provide an easily controlled means to regulate gene structure and function in living cells. Since recombination provides a stable and unambiguous record of protein uptake, the enzyme may also be used for quantitative studies of cis- and trans-acting factors that influence the delivery of proteins into cells.

Results

In the present study, 11 recombinant fusion proteins were analyzed to characterize sequences and conditions that affect protein uptake and/or activity and to develop more active cell-permeant enzymes. We report that the native enzyme has a low, but intrinsic ability to enter cells. The most active Cre proteins tested contained either an N-terminal 6xHis tag and a nuclear localization sequence from SV40 l

SUBMITTER: Lin Q 

PROVIDER: S-EPMC529453 | biostudies-literature | 2004 Oct

REPOSITORIES: biostudies-literature