Sequestration of Ribosome during Protein Aggregate Formation: Contribution of ribosomal RNA.

ABSTRACT: An understanding of the mechanisms underlying protein aggregation and cytotoxicity of the protein aggregates is crucial in the prevention of several diseases in humans. Ribosome, the cellular protein synthesis machine is capable of acting as a protein folding modulator. The peptidyltransferase center residing in the domain V of large ribosomal subunit 23S rRNA is the centre for the protein folding ability of the ribosome and is also the cellular target of several antiprion compounds. Our in vitro studies unexpectedly reveal that the partial unfolding or aggregation of lysozyme under reducing conditions in presence of the ribosome can induce aggregation of ribosomal components. Electrostatic interactions complemented by specific rRNA-protein interaction drive the ribosome-protein aggregatio

SUBMITTER: Pathak BK 

PROVIDER: S-EPMC5294636 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature