Transcriptome analysis reveals the complexity of alternative splicing regulation in the fungus Verticillium dahliae.

ABSTRACT:

Background

Alternative splicing (AS) regulation is extensive and shapes the functional complexity of higher organisms. However, the contribution of alternative splicing to fungal biology is not well studied.

Results

This study provides sequences of the transcriptomes of the plant wilt pathogen Verticillium dahliae, using two different strains and multiple methods for cDNA library preparations. We identified alternatively spliced mRNA isoforms in over a half of the multi-exonic fungal genes. Over one-thousand isoforms involve TopHat novel splice junction; multiple types of combinatory alternative splicing patterns were identified. We showed that one Verticillium gene could use four different 5' splice sites and two different 3' donor sites to produce up to five mature mRNAs,

SUBMITTER: Jin L 

PROVIDER: S-EPMC5294800 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature