Near-Atomic Three-Dimensional Mapping for Site-Specific Chemistry of 'Superbugs'.

ABSTRACT: Emergence of multidrug resistant Gram-negative bacteria has caused a global health crisis and last-line class of antibiotics such as polymyxins are increasingly used. The chemical composition at the cell surface plays a key role in antibiotic resistance. Unlike imaging the cellular ultrastructure with well-developed electron microscopy, the acquisition of a high-resolution chemical map of the bacterial surface still remains a technological challenge. In this study, we developed an atom probe tomography (APT) analysis approach to acquire mass spectra in the pulsed-voltage mode and reconstructed the 3D chemical distribution of atoms and molecules in the subcellular domain at the near-atomic scale. Using focused ion beam (FIB) milling together with micromanipulation, site-specific samples wer

SUBMITTER: Adineh VR 

PROVIDER: S-EPMC5295734 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature