Screening and Characterization of Drugs That Protect Corneal Endothelial Cells Against Unfolded Protein Response and Oxidative Stress.

ABSTRACT:

Purpose

To screen for and characterize compounds that protect corneal endothelial cells against unfolded protein response (UPR) and oxidative stress.

Methods

Bovine corneal endothelial cells (BCECs) were treated for 48 hours with 640 compounds from a Food and Drug Administration (FDA)-approved drug library and then challenged with thapsigargin or H2O2 to induce UPR or oxidative stress, respectively. Cell viability was measured using the CellTiter-Glo survival assay. Selected "hits" were subjected to further dose-response testing, and their ability to modulate expression of UPR and oxidative stress markers was assessed by RT-PCR, Western blot, and measurement of protein carbonyl and 8-hydroxydeoxyguanosine (8-OHdG) adducts in immortalized human corneal endothelial cells (iHCE

SUBMITTER: Kim EC 

PROVIDER: S-EPMC5295784 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature