Protein kinase Cɛ activity regulates mGluR5 surface expression in the rat nucleus accumbens.

ABSTRACT: Type 5 metabotropic glutamate receptors (mGluR5) activate protein kinase C (PKC) via coupling to G_αq/11 protein signaling. We have previously demonstrated that the epsilon isoform of PKC (PKCɛ) is a critical downstream target of mGluR5 in regulating behavioral and biochemical responses to alcohol. Recent evidence suggests that PKC-mediated phosphorylation of mGluR5 can lead to receptor desensitization and internalization. We therefore sought to examine the specific involvement of PKCɛ in the regulation of mGluR5 surface expression in the nucleus accumbens (NAc), a key regulator of alcohol-associated behaviors. Coronal brain sections from male Wistar rats were analyzed for either colocalization of mGluR5 and PKCɛ via immunohistochemistry or changes in mGluR5 surface expression an

SUBMITTER: Schwendt M 

PROVIDER: S-EPMC5296288 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature