Nucleosome Positioning and NDR Structure at RNA Polymerase III Promoters.

ABSTRACT: Chromatin is structurally involved in the transcriptional regulation of all genes. While the nucleosome positioning at RNA polymerase II (pol II) promoters has been extensively studied, less is known about the chromatin structure at pol III promoters in human cells. We use a high-resolution analysis to show substantial differences in chromatin structure of pol II and pol III promoters, and between subtypes of pol III genes. Notably, the nucleosome depleted region at the transcription start site of pol III genes extends past the termination sequences, resulting in nucleosome free gene bodies. The +1 nucleosome is located further downstream than at pol II genes and furthermore displays weak positioning. The variable position of the +1 location is seen not only within individual cell populati

SUBMITTER: Helbo AS 

PROVIDER: S-EPMC5296907 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature