AMPK regulates lipid accumulation in skeletal muscle cells through FTO-dependent demethylation of N⁶-methyladenosine.

ABSTRACT: Skeletal muscle plays important roles in whole-body energy homeostasis. Excessive skeletal muscle lipid accumulation is associated with some metabolic diseases such as obesity and Type 2 Diabetes. The energy sensor AMPK (AMP-activated protein kinase) is a key regulator of skeletal muscle lipid metabolism, but the precise regulatory mechanism remains to be elucidated. Here, we provide a novel mechanism by which AMPK regulates skeletal muscle lipid accumulation through fat mass and obesity-associated protein (FTO)-dependent demethylation of N⁶-methyladenosine (m⁶A). We confirmed an inverse correlation between AMPK and skeletal muscle lipid content. Moreover, inhibition of AMPK enhanced lipid accumulation, while activation of AMPK reduced lipid accumulation in skeletal m

SUBMITTER: Wu W 

PROVIDER: S-EPMC5296945 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature