Cloning, expression and molecular characterization of a Cystoisospora suis specific uncharacterized merozoite protein.

ABSTRACT:

Background

The genome of the apicomplexan parasite Cystoisospora suis (syn. Isospora suis) has recently been sequenced and annotated, opening the possibility for the identification of novel therapeutic targets against cystoisosporosis. It was previously proposed that a 42 kDa uncharacterized merozoite protein, encoded by gene CSUI_005805, might be a relevant vaccine candidate due to its high immunogenic score, high expression level and species-specificity as determined in silico.

Methods

The 1170 bp coding sequence of the CSUI_005805 gene was PCR amplified and cloned into the bacterial expression vector pQE-31. The specificity of the expressed recombinant protein was evaluated in an immunoblot, and relative levels of expression in different developmental stages and subcellul

SUBMITTER: Shrestha A 

PROVIDER: S-EPMC5297187 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature