Ser1928 phosphorylation by PKA stimulates the L-type Ca2+ channel CaV1.2 and vasoconstriction during acute hyperglycemia and diabetes.

ABSTRACT: Hypercontractility of arterial myocytes and enhanced vascular tone during diabetes are, in part, attributed to the effects of increased glucose (hyperglycemia) on L-type Ca_V1.2 channels. In murine arterial myocytes, kinase-dependent mechanisms mediate the increase in Ca_V1.2 activity in response to increased extracellular glucose. We identified a subpopulation of the Ca_V1.2 channel pore-forming subunit (α1_C) within nanometer proximity of protein kinase A (PKA) at the sarcolemma of murine and human arterial myocytes. This arrangement depended upon scaffolding of PKA by an A-kinase anchoring protein 150 (AKAP150) in mice. Glucose-mediated increases in Ca_V1.2 channel activity were associated with PKA activity, leading to α1_C pho

SUBMITTER: Nystoriak MA 

PROVIDER: S-EPMC5297430 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature