Efficient production of bispecific IgG of different isotypes and species of origin in single mammalian cells.

ABSTRACT: Bispecific IgG production in single host cells has been a much sought-after goal to support the clinical development of these complex molecules. Current routes to single cell production of bispecific IgG include engineering heavy chains for heterodimerization and redesign of Fab arms for selective pairing of cognate heavy and light chains. Here, we describe novel designs to facilitate selective Fab arm assembly in conjunction with previously described knobs-into-holes mutations for preferential heavy chain heterodimerization. The top Fab designs for selective pairing, namely variants v10 and v11, support near quantitative assembly of bispecific IgG in single cells for multiple different antibody pairs as judged by high-resolution mass spectrometry. Single-cell and in vitro-assembled bispec

SUBMITTER: Dillon M 

PROVIDER: S-EPMC5297516 | biostudies-literature | 2017 Feb/Mar

REPOSITORIES: biostudies-literature