Anti-Neuroblastoma Properties of a Recombinant Sunflower Lectin.

ABSTRACT: According to their sugar recognition specificity, plant lectins are proposed as bioactive proteins with potential in cancer treatment and diagnosis. Helja is a mannose-specific jacalin-like lectin from sunflower which was shown to inhibit the growth of certain fungi. Here, we report its recombinant expression in a prokaryotic system and its activity in neurobalstoma cells. Helja coding sequence was fused to the pET-32 EK/LIC, the enterokinase/Ligation-independent cloning vector and a 35 kDa protein was obtained in Escherichia coli representing Helja coupled to thioredoxin (Trx). The identity of this protein was verified using anti-Helja antibodies. This chimera, named Trx-rHelja, was enriched in the soluble bacterial extracts and was purified using Ni⁺²-Sepharose and d-ma

SUBMITTER: Pinedo M 

PROVIDER: S-EPMC5297726 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature