Guidelines for the successful generation of protein-ligand complex crystals.

ABSTRACT: With continuous technical improvements at synchrotron facilities, data-collection rates have increased dramatically. This makes it possible to collect diffraction data for hundreds of protein-ligand complexes within a day, provided that a suitable crystal system is at hand. However, developing a suitable crystal system can prove challenging, exceeding the timescale of data collection by several orders of magnitude. Firstly, a useful crystallization construct of the protein of interest needs to be chosen and its expression and purification optimized, before screening for suitable crystallization and soaking conditions can start. This article reviews recent publications analysing large data sets of crystallization trials, with the aim of identifying factors that do or do not make a good crys

SUBMITTER: Muller I 

PROVIDER: S-EPMC5297911 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature