MnO₂ nanosheet mediated "DD-A" FRET binary probes for sensitive detection of intracellular mRNA.

ABSTRACT: The donor donor-acceptor (DD-A) FRET model has proven to have a higher FRET efficiency than donor-acceptor acceptor (D-AA), donor-acceptor (D-A), and donor donor-acceptor acceptor (DD-AA) FRET models. The in-tube and in-cell experiments clearly demonstrate that the "DD-A" FRET binary probes can indeed increase the FRET efficiency and provide higher imaging contrast, which is about one order of magnitude higher than the ordinary "D-A" model. Furthermore, MnO₂ nanosheets were employed to deliver these probes into living cells for intracellular TK1 mRNA detection because they can adsorb ssDNA probes, penetrate across the cell membrane and be reduced to Mn²⁺ ions by intracellular GSH. The results indicated that the MnO₂ nanosheet mediated "DD-A" FRET binary pro

SUBMITTER: Ou M 

PROVIDER: S-EPMC5297934 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature