Peptide de novo sequencing of mixture tandem mass spectra.

ABSTRACT: The impact of mixture spectra deconvolution on the performance of four popular de novo sequencing programs was tested using artificially constructed mixture spectra as well as experimental proteomics data. Mixture fragmentation spectra are recognized as a limitation in proteomics because they decrease the identification performance using database search engines. De novo sequencing approaches are expected to be even more sensitive to the reduction in mass spectrum quality resulting from peptide precursor co-isolation and thus prone to false identifications. The deconvolution approach matched complementary b-, y-ions to each precursor peptide mass, which allowed the creation of virtual spectra containing sequence specific fragment ions of each co-isolated peptide. Deconvolution processing re

SUBMITTER: Gorshkov V 

PROVIDER: S-EPMC5297990 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature