Biosurfactant and Degradative Enzymes Mediated Crude Oil Degradation by Bacterium Bacillus subtilis A1.

ABSTRACT: In this work, the biodegradation of the crude oil by the potential biosurfactant producing Bacillus subtilis A1 was investigated. The isolate had the ability to synthesize degradative enzymes such as alkane hydroxylase and alcohol dehydrogenase at the time of biodegradation of hydrocarbon. The biosurfactant producing conditions were optimized as pH 7.0, temperature 40°C, 2% sucrose and 3% of yeast extract as best carbon and nitrogen sources for maximum production of biosurfactant (4.85 g l^-1). Specifically, the low molecular weight compounds, i.e., C₁₀-C₁₄ were completely degraded, while C₁₅-C₁₉ were degraded up to 97% from the total hydrocarbon pools. Overall crude oil degradation efficiency of the strain A1 was about 87% withi

SUBMITTER: Parthipan P 

PROVIDER: S-EPMC5299021 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature