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An air-liquid interphase approach for modeling the early embryo-maternal contact zone.

ABSTRACT: We developed an air-liquid interphase culture procedure for mammalian oviduct epithelial cells leading to the formation of functional epithelial tissues, which generate oviduct fluid surrogates. These in vitro oviduct epithelia can be co-cultured with living zygotes and enable embryonic development up to the blastocyst stage without addition of embryo culture medium. The described strategy is broadly applicable to analyze early embryo-maternal interactions under standardized in vitro conditions.

SUBMITTER: Chen S

PROVIDER: S-EPMC5299422 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature

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An air-liquid interphase approach for modeling the early embryo-maternal contact zone.

Chen S S Palma-Vera S E SE Langhammer M M Galuska S P SP Braun B C BC Krause E E Lucas-Hahn A A Schoen J J

Scientific reports 20170209

We developed an air-liquid interphase culture procedure for mammalian oviduct epithelial cells leading to the formation of functional epithelial tissues, which generate oviduct fluid surrogates. These in vitro oviduct epithelia can be co-cultured with living zygotes and enable embryonic development up to the blastocyst stage without addition of embryo culture medium. The described strategy is broadly applicable to analyze early embryo-maternal interactions under standardized in vitro conditions. ...[more]

PMID: 28181558

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