Cyclic AMP reverses the effects of aging on pacemaker activity and If in sinoatrial node myocytes.

ABSTRACT: Aerobic capacity decreases with age, in part because of an age-dependent decline in maximum heart rate (mHR) and a reduction in the intrinsic pacemaker activity of the sinoatrial node of the heart. Isolated sinoatrial node myocytes (SAMs) from aged mice have slower spontaneous action potential (AP) firing rates and a hyperpolarizing shift in the voltage dependence of activation of the "funny current," I_f Cyclic AMP (cAMP) is a critical modulator of both AP firing rate and I_f in SAMs. Here, we test the ability of endogenous and exogenous cAMP to overcome age-dependent changes in acutely isolated murine SAMs. We found that maximal stimulation of endogenous cAMP with 3-isobutyl-1-methylxanthine (IBMX) and forskolin significantly increased AP firing rate and depolarized t

SUBMITTER: Sharpe EJ 

PROVIDER: S-EPMC5299620 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature