Optimization of DNA extraction for advancing coral microbiota investigations.

ABSTRACT:

Background

DNA-based sequencing approaches are commonly used to identify microorganisms and their genes and document trends in microbial community diversity in environmental samples. However, extraction of microbial DNA from complex environmental samples like corals can be technically challenging, and extraction methods may impart biases on microbial community structure.

Methods

We designed a two-phase study in order to propose a comprehensive and efficient method for DNA extraction from microbial cells present in corals and investigate if extraction method influences microbial community composition. During phase I, total DNA was extracted from seven coral species in a replicated experimental design using four different MO BIO Laboratories, Inc., DNA Isolation kits: PowerSoi

SUBMITTER: Weber L 

PROVIDER: S-EPMC5299696 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature