Error baseline rates of five sample preparation methods used to characterize RNA virus populations.

ABSTRACT: Individual RNA viruses typically occur as populations of genomes that differ slightly from each other due to mutations introduced by the error-prone viral polymerase. Understanding the variability of RNA virus genome populations is critical for understanding virus evolution because individual mutant genomes may gain evolutionary selective advantages and give rise to dominant subpopulations, possibly even leading to the emergence of viruses resistant to medical countermeasures. Reverse transcription of virus genome populations followed by next-generation sequencing is the only available method to characterize variation for RNA viruses. However, both steps may lead to the introduction of artificial mutations, thereby skewing the data. To better understand how such errors are introduced durin

SUBMITTER: Kugelman JR 

PROVIDER: S-EPMC5300104 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature