Genetic and functional characterisation of the lactococcal P335 phage-host interactions.

ABSTRACT:

Background

Despite continuous research efforts, bacterio(phages) infecting Lactococcus lactis starter strains persist as a major threat to dairy fermentations. The lactococcal P335 phages, which are currently classified into four sub-groups (I-IV), are the second most frequently isolated phage group in an industrial dairy context.

Results

The current work describes the isolation and comparative genomic analysis of 17 novel P335 group phages. Detailed analysis of the genomic region of P335 phages encoding the so-called "baseplate", which includes the receptor binding protein (RBP) was combined with a functional characterization of the RBP of sub-group III and IV phages. Additionally, calcium-dependence assays revealed a specific requirement for calcium by sub-group IV phages

SUBMITTER: Mahony J 

PROVIDER: S-EPMC5301393 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature