Comparative and Quantitative Global Proteomics Approaches: An Overview.

ABSTRACT: Proteomics became a key tool for the study of biological systems. The comparison between two different physiological states allows unravelling the cellular and molecular mechanisms involved in a biological process. Proteomics can confirm the presence of proteins suggested by their mRNA content and provides a direct measure of the quantity present in a cell. Global and targeted proteomics strategies can be applied. Targeted proteomics strategies limit the number of features that will be monitored and then optimise the methods to obtain the highest sensitivity and throughput for a huge amount of samples. The advantage of global proteomics strategies is that no hypothesis is required, other than a measurable difference in one or more protein species between the samples. Global proteomics meth

SUBMITTER: Deracinois B 

PROVIDER: S-EPMC5302699 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature