Quantitative Proteomic Profiling of Low-Dose Ionizing Radiation Effects in a Human Skin Model.

ABSTRACT: To assess responses to low-dose ionizing radiation (LD-IR) exposures potentially encountered during medical diagnostic procedures, nuclear accidents or terrorist acts, a quantitative proteomic approach was used to identify changes in protein abundance in a reconstituted human skin tissue model treated with 0.1 Gy of ionizing radiation. To improve the dynamic range of the assay, subcellular fractionation was employed to remove highly abundant structural proteins and to provide insight into radiation-induced alterations in protein localization. Relative peptide quantification across cellular fractions, control and irradiated samples was performing using 8-plex iTRAQ labeling followed by online two-dimensional nano-scale liquid chromatography and high resolution MS/MS analysis. A total of 107

SUBMITTER: Hengel SM 

PROVIDER: S-EPMC5302749 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature