Metabolomic tool to identify soybean [Glycine max (L.) Merrill] germplasms with a high level of shade tolerance at the seedling stage.

ABSTRACT: The isoflavone profiles of seeds of various soybean genotypes with different levels of shade tolerance at the seedling stage were investigated. High-performance liquid chromatography (HPLC) was used to quantify 12 isoflavones, and the data were analyzed using a multivariate statistical analysis. Combined with field experimental data and an orthogonal partial least-squares discriminant analysis (OPLS-DA), several aglycones (genistein (GE), daidzein (DE), and glycitein (GLE)) were selected and identified as key compounds involved in the shade tolerance of soybean seedlings. Additional correlation analysis and laboratory shading stress experiments with soybean seedlings also confirmed the function of these selected isoflavones, especially GE, in the discrimination of soybean seedlings with di

SUBMITTER: Liu J 

PROVIDER: S-EPMC5304147 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature