¹²⁵I-F56 Peptide as Radioanalysis Agent Targeting VEGFR1 in Mice Xenografted with Human Gastric Tumor.

ABSTRACT: ¹²⁵I-Radiolabeled F56 peptide was designed as a radioactive analogue of F56 (peptide WHSDMEWWYLLG) to bind with VEGFR1 receptor. It was synthesized in high radiochemical yield and specific activity. The in vitro stability of ¹²⁵I-F56 was tested, and the bioactivity of ¹²⁵I-F56 was confirmed by both cell uptake and binding affinity measurement in VEGFR1 positive BGC-823 cells. The time-radioactivity relationship and biodistribution of ¹²⁵I-F56 tracer were conducted using nude mice bearing human gastric carcinoma BGC-823, by noninvasive micro-SPECT/CT imaging. The tracer's tumor uptake was further confirmed by autoradiography and HE stain of ¹²⁵I-F56 in tumor tissues ex vivo. Those results demonstrated that ¹²⁵I-F56 hol

SUBMITTER: Zhu H 

PROVIDER: S-EPMC5304288 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature