CD8⁺ T Cells Utilize Highly Dynamic Enhancer Repertoires and Regulatory Circuitry in Response to Infections.

ABSTRACT: Differentiation of effector and memory CD8⁺ T cells is accompanied by extensive changes in the transcriptome and histone modifications at gene promoters; however, the enhancer repertoire and associated gene regulatory networks are poorly defined. Using histone mark chromatin immunoprecipitation coupled with deep sequencing, we mapped the enhancer and super-enhancer landscapes in antigen-specific naive, differentiated effector, and central memory CD8⁺ T cells during LCMV infection. Epigenomics-based annotation revealed a highly dynamic repertoire of enhancers, which were inherited, de novo activated, decommissioned and re-activated during CD8⁺ T cell responses. We employed a computational algorithm to pair enhancers with target gene promoters. On average, ea

SUBMITTER: He B 

PROVIDER: S-EPMC5304416 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature