Sexual pheromone modulates the frequency of cytosolic Ca²⁺ bursts in Saccharomyces cerevisiae.

ABSTRACT: Transient and highly regulated elevations of cytosolic Ca²⁺ control a variety of cellular processes. Bulk measurements using radioactive Ca²⁺ and the luminescent sensor aequorin have shown that in response to pheromone, budding yeast cells undergo a rise of cytosolic Ca²⁺ that is mediated by two import systems composed of the Mid1-Cch1-Ecm7 protein complex and the Fig1 protein. Although this response has been widely studied, there is no treatment of Ca²⁺ dynamics at the single-cell level. Here, using protein calcium indicators, we show that both vegetative and pheromone-treated yeast cells exhibit discrete and asynchronous Ca²⁺ bursts. Most bursts reach maximal amplitude in 1-10 s, range between 7 and 30 s, and decay in a way that fits

SUBMITTER: Carbo N 

PROVIDER: S-EPMC5305257 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature