Harvesting pre-polarized macrophages using thermo-responsive substrates.

ABSTRACT: In the cell culture environment macrophages are highly adherent cells. Currently used methods to harvest macrophages have the disadvantage of reducing cell viability and their ability to re-attach after seeding. Although thermo-responsive surfaces have been employed to harvest cell sheets no reports are available to use these to harvest (pre-polarized) macrophages. We show that this method significantly improves the yield of living macrophages and percentage of subsequent cell reattachment, whilst having a minimal effect on the cell phenotype.

SUBMITTER: Malheiro V 

PROVIDER: S-EPMC5307341 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature