A novel point mutation in RpoB improves osmotolerance and succinic acid production in Escherichia coli.

ABSTRACT:

Background

Escherichia coli suffer from osmotic stress during succinic acid (SA) production, which reduces the performance of this microbial factory.

Results

Here, we report that a point mutation leading to a single amino acid change (D654Y) within the β-subunit of DNA-dependent RNA polymerase (RpoB) significantly improved the osmotolerance of E. coli. Importation of the D654Y mutation of RpoB into the parental strain, Suc-T110, increased cell growth and SA production by more than 40% compared to that of the control under high glucose osmolality. The transcriptome profile, determined by RNA-sequencing, showed two distinct stress responses elicited by the mutated RpoB that counterbalanced the osmotic stress. Under non-stressed conditions, genes involved in the synthesis and t

SUBMITTER: Xiao M 

PROVIDER: S-EPMC5307762 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature