Targeted gene knock-in by CRISPR/Cas ribonucleoproteins in porcine zygotes.

ABSTRACT: The domestic pig is an important "dual purpose" animal model for agricultural and biomedical applications. There is an emerging consensus in the biomedical community for the use of large animal models such as pigs to either serve as an alternative, or complement investigations from the mouse. However, the use of pig has not proven popular due to technical difficulties and time required in generating models with desired genetic modifications. In this regard, the ability to directly modify the genome in the zygote and generate edited animals is highly desirable. This report demonstrates for the first time, the generation of gene targeted animals by direct injection of Cas9 ribonucleoprotein complex and short stretches of DNA sequences into porcine zygotes. The Cas9 protein from Streptococcus

SUBMITTER: Park KE 

PROVIDER: S-EPMC5307959 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature