How Native and Alien Metal Cations Bind ATP: Implications for Lithium as a Therapeutic Agent.

ABSTRACT: Adenosine triphosphate (ATP), the major energy currency of the cell, exists in solution mostly as ATP-Mg. Recent experiments suggest that Mg²⁺ interacts with the highly charged ATP triphosphate group and Li⁺ can co-bind with the native Mg²⁺ to form ATP-Mg-Li and modulate the neuronal purine receptor response. However, it is unclear how the negatively charged ATP triphosphate group binds Mg²⁺ and Li⁺ (i.e. which phosphate group(s) bind Mg²⁺/Li⁺) and how the ATP solution conformation depends on the type of metal cation and the metal-binding mode. Here, we reveal the preferred ATP-binding mode of Mg²⁺/Li⁺ alone and combined: Mg²⁺ prefers to bind ATP tridentately to each of the three p

SUBMITTER: Dudev T 

PROVIDER: S-EPMC5307966 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature