Specific membrane capacitance, cytoplasm conductivity and instantaneous Young's modulus of single tumour cells.

ABSTRACT: As label-free biomarkers, biophysical properties of cells are widely used for cell type classification. However, intrinsic biophysical markers, e.g., specific membrane capacitance (C_{specific membrane}), cytoplasm conductivity (σ_conductivity) and instantaneous Young's modulus (E_{instantaneous}) measured for hundreds of single cells were not yet reported. In this study, single cells in suspension (adherent cells treated with trypsin) were aspirated through a microfluidic constriction channel at 25 °C, and the entry processes and impedance profiles were recorded and translated to C_{specific membrane}, σ_conductivity and E_{instantaneous}. C_{specific membrane}, σ_conductivity and E_{instantaneous} of five cell

SUBMITTER: Wang K 

PROVIDER: S-EPMC5308201 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature